Halogenated Salts as Coagulant to Prepare Bovine Serum Albumin Nanoparticles Containing Paclitaxel using High-Pressure Homogenisation Method

In this study, bovine serum albumin-paclitaxel nanoparticles were prepared by high-pressure homogenization method with halogenated salt as coagulant. The effect of process parameters such albumin concentration, coagulant homogenisation time, pressure, water/ethanol ratio and was analyzed to optimize nanoparticle size, conversion rate encapsulation efficiency. Bovine concentration pressure found exert a great influence on particle size. Meanwhile, the significantly affected Electron microscopy showed that freeze-dried particles mostly existed in form dimers trimers an average size 300-400 nm. Infrared spectroscopy indicated paclitaxel well encapsulated albumin. Raman spectra synthesized changes disulphide bond configuration protein structure. addition, different crosslinking agents (genipin, vanillin glutaraldehyde) drug release from vitro conditions investigated. study confirmed had better sustained effect. These results suggest salts is effective prepare drug-delivery systems carrier.